Journal: Theranostics

Article Title: Targeting the Notch and TGF-β signaling pathways to prevent retinal fibrosis in vitro and in vivo

doi: 10.7150/thno.45192

Figure Lengend Snippet: Antibodies used for Western blots

Article Snippet: α-SMA , Cell Signaling#48938 , Rabbit , 1:1000.

Techniques: Western Blot

Journal: Theranostics

Article Title: Targeting the Notch and TGF-β signaling pathways to prevent retinal fibrosis in vitro and in vivo

doi: 10.7150/thno.45192

Figure Lengend Snippet: Intravitreal injection of RO4929097 inhibits overexpression of ECM proteins resulting from chemically-induced retinal damage. (A-E): Western blot analyses of ECM proteins including fibronectin (A) , integrin α5 (B) , MMP2 (C) and α-SMA (D) using retinal proteins. **P<0.01 and ****P<0.0001, vs normal mice receiving vehicle. † P<0.05, and ‡ P<0.01, paired t-tests between RO and Ve treated groups. N=4-7/group. Multiple comparison corrections were calculated using one-way ANOVA followed by Tukey's multiple comparison test, P < 0.0001 in (A) , P = 0.002 in (B) , P = 0.002 in (C) , P = 0.004 in (D) . Nor/Vehicle, normal eye receiving vehicle injection.

Article Snippet: α-SMA , Cell Signaling#48938 , Rabbit , 1:1000.

Techniques: Injection, Over Expression, Western Blot

Journal: Nature Communications

Article Title: CD248-targeted BBIR-T cell therapy against late-activated fibroblasts in cardiac repair after myocardial infarction

doi: 10.1038/s41467-025-56703-2

Figure Lengend Snippet: a UMAP clustering of 13441 cardiac interstitial cells (left) and fibroblasts (right) isolated from C57BL/6 J wild-type (WT) mice at 7 and 14 days post-sham and post-MI surgery. Each dot represents a single cell. The expression of known marker genes annotated cell type. b UMAP and violin plots depict Postn , Acta2 , and Comp expression in fibroblasts. c Triple IF staining for periostin (green) and αSMA (red) in the peri-infarct zone of the hearts of C57BL/6 J WT mice 0, 7, 14, 21, and 28 days after MI surgery. Nuclei were stained blue. Scale bar = 100 μm. Quantification of the number of periostin + αSMA + F-Myo and periostin + αSMA - F-Act in each high-power field (HPF). n = 5 mice at each time point after MI. d Flow cytometry of fibroblasts isolated from hearts of C57BL/6 J WT and Pdgfra-CRE:tdTomato mice. Total cardiac fibroblasts were lineage traced by pdgfrα. F-Myo and F-Act were marked with antibodies against periostin-AF488 and αSMA-AF647. Quantification of the pdgfrα + fibroblast ratio in live cells, periostin + fibroblasts ratio in pdgfrα + fibroblasts, periostin + αSMA + F-Myo ratio in pdgfrα + fibroblasts, and periostin + αSMA - F-Act ratio in pdgfrα + fibroblasts. n = 5 mice at each time point after MI. e Spatial distribution of the F-Act and F-Myo subpopulation in mouse heart sections from sham and 10 days post-MI conditions, visualized through the integration of scRNA-seq and stereo-seq methodologies. c, d Data are the mean ± SEM and p -values are displayed in the bar charts, one-way ANOVA followed by Tukey’s multiple comparisons tests. Source data are provided as a Source Data file.

Article Snippet: Primary antibodies were Periostin (1:200, Abcam, ab14041), αSMA (1:200, Cell Signaling, 19245 and 48938), CD248 (1:1000, Santa Cruz, sc-377221), COMP (1:1000, Genetex, GTX14515), CD31 (1:200, BD Biosciences, 550300), Ly6G (1:200, Abcam, ab25377), CD68 (1:200, Abcam, ab955), and CD11b (1:200, BD Biosciences, 550282).

Techniques: Isolation, Expressing, Marker, Staining, Flow Cytometry

Journal: Nature Communications

Article Title: CD248-targeted BBIR-T cell therapy against late-activated fibroblasts in cardiac repair after myocardial infarction

doi: 10.1038/s41467-025-56703-2

Figure Lengend Snippet: a Triple IF staining for periostin (green) and CD248 (red) in the peri-infarct zone of the hearts of C57BL/6J WT mice 0, 7, 14, 21, and 28 days after MI surgery. Nuclei were stained blue. Scale bar = 100 μm. Quantification of the periostin + CD248 + cell to DAPI ratio in each HPF. n = 5 mice at each time point after MI. Triple IF staining of periostin, αSMA, COMP (stained green), and CD248 (stained red) in the hearts of MI mouses ( b ) and a patient with ischemic cardiomyopathy ( c ). Nuclei were stained blue. Scale bar =100 μm. IF staining of mouse heart was repeated in heart sections from 5 independent mice, while IF of human heart was repeated 5 times in heart sections from one patient with ischemic cardiomyopathy, yielding similar results. d Flow cytometry of cells isolated from hearts of C57BL/6 J WT mouses and Pdgfra-CRE:tdTomato mice. Total cardiac fibroblasts were lineage traced by pdgfrα. Quantification of the CD248 + cell ratio in live cells, pdgfrα + fibroblast ratio in CD248 + cells, CD248 + cell ratio in pdgfrα + fibroblasts, periostin + CD248 + cell ratio in pdgfrα + fibroblasts, CD248 + cell ratio in periostin + activated fibroblasts, and periostin + activated fibroblasts in CD248 + cells. n = 5 mice at each time point after MI. a, d Data are the mean ± SEM and p -values are displayed in the bar charts, one-way ANOVA followed by Tukey’s multiple comparisons tests. Source data are provided as a Source Data file.

Article Snippet: Primary antibodies were Periostin (1:200, Abcam, ab14041), αSMA (1:200, Cell Signaling, 19245 and 48938), CD248 (1:1000, Santa Cruz, sc-377221), COMP (1:1000, Genetex, GTX14515), CD31 (1:200, BD Biosciences, 550300), Ly6G (1:200, Abcam, ab25377), CD68 (1:200, Abcam, ab955), and CD11b (1:200, BD Biosciences, 550282).

Techniques: Staining, Flow Cytometry, Isolation

Journal: Nature Communications

Article Title: CD248-targeted BBIR-T cell therapy against late-activated fibroblasts in cardiac repair after myocardial infarction

doi: 10.1038/s41467-025-56703-2

Figure Lengend Snippet: a Schematic diagram of the structure of the BBIR-T system. Created in BioRender. Haiting, C. (2025) https://BioRender.com/l46n352 . b Schematic images of dcAv. BBIR-28-137ζ and CD19. CAR-137ζ sequence. c Western blotting shows exogenous CD3ζ expression in HEK-293T cells transduced with lentivirus (LV)-dcAv.BBIR and LV-anti-CD19.CAR. The samples derive from the same experiment and that gels/blots were processed in parallel. Quantifying the relative protein expression of CD3ζ to GAPDH. n = 4 cell culture wells of each group. d Flow cytometry of human peripheral T cells transduced with LV-dcAv.BBIR and LV-anti-CD19.CAR. Quantifying GFP + cells ratio to total T cells and biotin + cells ratio to GFP + cells. n = 5 cell culture wells of each group. e Western blotting by BF shows CD248 expression in NIH-3T3 cells with or without Cd248 overexpression(OE) or knockout(KO). The samples were derived from the same experiment and that gels/blots were processed in parallel. Quantifying the relative protein expression of CD248 to GAPDH. n = 4 cell culture wells of each group. f Triple IF staining of periostin, αSMA, COMP (green), and biotinylated anti-CD248 F(ab’)2 (shorted as BF) (red) in the peri-infarct zone 14 days after MI surgery. Nuclei were stained blue. Scale bar =100 μm. Each experiment was repeated 5 times independently with similar results. c-e Data are the mean ± SEM and p -values are displayed in the bar charts, one-way ANOVA followed by Tukey’s multiple comparisons tests. Source data are provided as a Source Data file.

Article Snippet: Primary antibodies were Periostin (1:200, Abcam, ab14041), αSMA (1:200, Cell Signaling, 19245 and 48938), CD248 (1:1000, Santa Cruz, sc-377221), COMP (1:1000, Genetex, GTX14515), CD31 (1:200, BD Biosciences, 550300), Ly6G (1:200, Abcam, ab25377), CD68 (1:200, Abcam, ab955), and CD11b (1:200, BD Biosciences, 550282).

Techniques: Sequencing, Western Blot, Expressing, Transduction, Cell Culture, Flow Cytometry, Over Expression, Knock-Out, Derivative Assay, Staining

Journal: Stem Cell Research & Therapy

Article Title: MSC-derived exosomes ameliorate erectile dysfunction by alleviation of corpus cavernosum smooth muscle apoptosis in a rat model of cavernous nerve injury

doi: 10.1186/s13287-018-1003-1

Figure Lengend Snippet: Neuronal nitric oxide synthase (nNOS) and caspase-3 expression in the cavernosum. a Representative immunofluorescence staining of nNOS ( green ) and TUNEL ( red ) in a penile midshaft specimen, 4 weeks after CNI and treatment (nNOS positive cells— green arrowheads , TUNEL positive nuclei— red arrowheads , nNOS and TUNEL both positive cells— white arrowheads ). b Representative images of western blots for nNOS in cavernosum from each group. Original magnification, ×400. DAPI = 4′,6-diamidino-2-phenylindole. c Data are presented as the relative density of nNOS compared with that of β-actin. d Representative images of western blots for caspase-3 in cavernosum from each group. e Data are presented as the relative density of caspase-3 compared with that of β-actin. Each bar depicts the means ± standard deviation from n = 8 animals per group. * p < 0.05 compared with the PBS vehicle group

Article Snippet: The penile segment sections were incubated with primary antibodies to anti-neuronal nitric oxide synthase (Cell Signaling Technology Co., Ltd., Danvers, MA, USA, #4231, 1:200) and anti-smooth muscle actin (Cell Signaling Technology Co., Ltd., #48938, 1:500).

Techniques: Expressing, Immunofluorescence, Staining, TUNEL Assay, Western Blot, Standard Deviation